A discussion on the adsorption effect of nano-selenium rich dark green tea in the treatment of 192 hepatitis B virus carriers
Qu Yuan Qu Lai Qu Shaozhong Huang Chixiong

Adsorption is a phenomenon that a phase of molecule or atom of matter can adhere to another phase of the matter. The substance that adheres to another is called adsorbent and that being adhered is called adsorbate.

Matter has adsorptive power because the molecules at the boundary of two phases stand in a specific state. There is a strong attracting power between the molecules in a matter phase, the magnitude of which depends on the summation of the gravitation of chemical bond between adjacent molecules in a material phase and Van der waal's attraction force between molecules. In the course of adsorption, the number of adsorbates enlarges with the increase of the surface of adsorbent. Therefore, in order to achieve a greater adsorptive effect, it is essential to maximize the surface of adsorbent by decreasing the size and increasing the surface area of particles. The adsorption effect of Nano-particles has attracted special attention from more and more researchers in the field of biomedicine because of its small grain diameter and large specific surface area.

• The best bio-adsorption plant - Nano-tea

Tea is a matter with excellent adsorption and safe to take, and the dissoluble fibre, protein, vitamin and mineral substance in the tea are essential to human body.

Tea, with its strong adsorptivity, can absorb moisture in the air as well as other peculiar smell in gases, and so that tea can be processed into deodoriferant, fishy odor removing agent and metal and waste gas adsorbent to be used in the course of waste-water treatment with good efficiency. After crushed into nano-grade with a maximal surface area, for example, a kilogram of nano-tea having a surface area of several square meters, the tea can have a strong adsorption effect.

The variant adsorption action of tea is expressed in physical form under normal and low temperature and in chemical form under high temperature, and depends on the unit surface of tea, the greater the unit surface, the greater the adsorptive power. In accordance with this principle, we have adopted nano-technology to increase the surface area of tea by a large margin. The surface of tea varies with its hole sizes, hole distribution and porosity, and thus tea has different adsorptive action. Generally speaking, the tea prepared with tender leaves has much more and smaller surfaces and inner holes and thus stronger adsorption, while the tea prepared with old leaves has less and lager surfaces and inner holes, and thus weaker adsorption. Therefore, we have adopted partially the first grade tender leaves to make the nano-tea fragrant in smell and strong in adsorption, and partially the old leaves (having high contents of selenium) to strengthen the selenium supplementing effect of nano-tea.

The effect of nano-selenium rich dark green tea in resisting viruses should not be neglected (See "Nano-science and technology" No.2, 2005).

• Application of adsorption effect to the resistance of viruses

2.1 The primary adsorption method for resistance of viruses

Early in 1980s, the adsorption effect was used to resist viruses. For example, Ding firstly used active carbon to adsorb viruses and then used cathartic to eliminate toxic in treating viral hepatitis with good results; At the begging of 1990s, Qu made use mainly of loach carbon processed into micro-grade to adsorb viruses, also showing an outstanding effect. To increase the adsorption efficiency, the materials should be crushed again 10 times fine, namely to the size equivalent to that of viruses. However this would make the carbon (active carbon) unsafe to take, because carbon is dissolvable and once entering the blood and bowel, it is difficult to be entirely discharged and discharging part of it will give much trouble to the kidney. For this reason, tea among the variety of plants has received special attention.

The adsorbability of tea is decided by its physical and biochemical characteristics. The spongy tissue of tea is well developed, and the tender leaves of tea have a high concentration of water. Therefore, the dried tea, with high porosity, is of a soft and multi-holes structure, manifested not only in its outside shape, but also in its complicate inner surface, with many small holes running through the entire leave and connecting the outside. With the inner surfaces of a great number of hole channels added up, the total effective area of a leave is very large, and the "hovering valence" has a large attractive power, and these decide the characteristics of tea in adsorption of variants.

Tea, after crushed to nano-grade and thus having a large molecular weight and a complex structure, enjoys a strong acting force and adsorption power between molecules, which also belong to the scope of adsorption generated by Van der Waal's attraction force. The excellent adsorption of tea is related with the chemical substances in leaves of tea. The leave of tea contains considerable soft colloid and chemical components, such as palmitinic acid of fatty group, terpenes and dibutyl phthalate, which belong to high boiling point substances and can adsorb and fix variants. Viruses, after adsorbed by tea will lose their activity.

2.2 Discussion on the adsorption effect of nano-selenium rich dark green tea in the treatment of 192 hepatitis B virus carriers

192 cases (male 112, female 80) of hepatitis B virus carriers who have taken nano-selenium green tea have been followed up. All of them belong to sub-healthy people with 102 cases of large three yang, 69 cases of small three yang and 21 case of others, and they have taken nano-tea for three months to one year..

The nano-selenium dark green, provided by Qinhuangdao Taiji Ring Nano-Products Co., Ltd, has a grain diameter averaging 100nm and distributed between 10-200nm. For persons with normal body weight, it has been taken 2.5g a time and 2-3 times a day after mixed with cold or hot boiled water and stirred with "tea stirring method".

Nano-selenium rich dark green tea has significant antigen effects against hepatitis B viruses (See Table 1).

Table 1 Antigen effects of nano-selenium dark green tea against hepatitis B virus carriers



Surface antigen (HBSAg)

e antigen (HBcAg)

Core antigen (anti-HBC)


Number of yang transformed to yin

Rate of yang transformed to yin


Number of yang transformed to yin

Rate of yang transformed to yin


Number of yang transformed to yin

Rate of yang transformed to yin

Effect of nano-dark green tea










As seen from the above table, the rate of yang transformed to yin of surface antigen (HBSAg) is 41%, that of e antigen (HBcAg) 71%, especially that of core antigen (anti-HBC), which has reached 75%. This indicates that some small particles might have penetrated into the core of viruses except its effect of absorbing and removing quite a number of viruses. This is an encouraging sign and worth further study.

• Application prosperity of adsorption effect in the field of biomedicine

In the next 5 years, the application of nano-adsorption effect will have a breakthrough in aspects of resisting viruses, and the most applicable will be multi-hole, organic and quick metabolized products, including nano-selenium with horn shape. The first to be adsorbed are hepatitis B and C viruses, followed by AIDA viruses. Hepatitis B virus is a kind of pantropic virus. It exists and duplicates itself in the tissues and cells of liver, bile duct, pancreas, lymph, etc. Generally speaking, hepatitis B viruses in the blood are easy to be removed by anti-HIV drugs, but those in histiocytes are not. This is the main reason why hepatitis is difficult to treat and easy to recur. Nano-particles assume a diffusion state, and with no membrane able to hamper their penetration, they can enter the tissues and cells of various organs of human body, and thus they can remove the viruses in all the organs and cells. Once there is a breakthrough in the study on hepatitis B viruses, it will bring along and promote the deepening of study on other viruses, and thus has wide application prospects.

The second to be adsorbed are cholesterin, free radicals and fat in the blood. The increase of these maters in human body may lead to the change of blood viscosity and the aging of blood vessels. The research now carried out by some scientists shows the particles of plants play a delicate role in adsorbing and removing these waste matters, purifying blood, resisting viruses and aging. These particles of plants (should be dissoluble, safe and innoxious) can be prepared either as drugs in forms of oral liquid, tablet and capsule, or as food in forms of drink, instant tea, wine and milk, making the routine food enjoy heath care functions and the reduction of cholesterin, free radicals, fat, blood sugar and avoirdupois merge into daily life.

The key to ensure the smooth progress and a great breakthrough in the study is dynamic observation facilities. Pathologic ultramicro-slice electron microscope can be used only in animal experiences, and for the study on the adsorption effect in human body, a living cell ultramicro-imaging technique is required. For this purpose, Shanghai Bukang Medical Instrument Co., Ltd has developed an ultramicroorganism imaging system, which can be used to observe the dynamic metabolism of living cells, with a resolving power near 200nm, only a step away from the capability of observing viruses and adsorption state. Now the company is striving to further increase the resolving power of the device, and with the aid of this device, the study on adsorption effect will take a great leap forward in the near future.

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